Journal: Oxidative Medicine and Cellular Longevity

Article Title: Resveratrol Mitigates Hippocampal Tau Acetylation and Cognitive Deficit by Activation SIRT1 in Aged Rats following Anesthesia and Surgery

doi: 10.1155/2020/4635163

Figure Lengend Snippet: Experimental protocol in vivo and in vitro. In vivo 21-month-old rats were pretreated with DMSO-saline solution or resveratrol via intraperitoneal injection for 7 consecutive days and received control gas or splenectomy under 3% sevoflurane for 2 h. After exposure, part of the rats was decapitated, and tissue samples were collected for biochemistry study, and part of the rats received the MWM test. In vitro BV2 cell was cultured and treated with DMSO-saline solution or resveratrol for 24 h, followed by treatment of control gas or LPS and 4% sevoflurane for 6 h. After exposure, conditioned medium were collected for primary neuron study, and tissue was collected for biochemistry study. After 7-day culture, primary neuron was incubated with four corresponding groups of conditioned medium for 24 h. Tissue was collected for biochemistry study after exposure.

Article Snippet: Then, another BV2 cell populations were allocated into four groups (Supplementary Figure ): (1) control+resveratrol, cells in the control group were treated with resveratrol (20 μ mol, Selleck Chemicals); (2) LPS+sevoflurane+resveratrol, cells in this group were treated with DMSO+LPS (100 ng/ml, Sigma-Aldrich, St. Louis, USA)+sevoflurane (4% for 6 hours)+resveratrol (20 μ mol); (3) resveratrol+EX527, cells in this group were treated with resveratrol (20 μ mol) + EX527 (100 nM, Selleck Chemicals); and (4) LPS+sevoflurane+resveratrol+EX527, cells in this group were treated with resveratrol (20 μ mol)+EX527 (100 nM)+LPS (100 ng/ml)+sevoflurane (4% for 6 hours).

Techniques: In Vivo, In Vitro, Injection, Cell Culture, Incubation